Terminal Deoxynucleotidyl Transferase (TdT) Mediated Synthesis of Protein Binding Aptamers

Nature has been constantly improving the functional properties of biomolecules for millions of years by introducing random mutations into the genetic makeup of life in a process known as evolution.  Only recently, has mankind been able to replicate this process in the lab through a technique called directed evolution.  Despite, the great promise of this technique, directed evolution is both labour intensive and costly.  It is therefore necessary to develop simplified and cheaper methodologies for directed evolution to allow its widespread adoption.  In this project we aim to utilise the unique properties of terminal deoxynucleotidyl transferase (TdT) enzyme to develop a rapid and cost effective non-evolutionary approach to develop protein binding ssDNA aptamers with strong binding properties.  If successful, the new approach will give us new insights into the possible mechanisms involved in the formation of early life and reshape our understanding of evolution.